A--AAA Validation of Polymorphisms Found from Whole Genome Sequencing

1. GENERAL SCOPE The contract is expected to begin in September 2016 for one (1) twelve (12) month base period. Nextera-based capture and sequencing. Validation of sequencing. Our initial whole genome sequencing work has identified several genetic polymorphisms associated with Abdominal Aortic Aneurysm (AAA). These variants are of different sizes, some of which are difficult to validate by traditional methods. Nextera-based library generation will provide our best chance for true validation as Nextera enrichment provides better quality mid-to-large size fragments. 2. CONTRACTOR QUALIFICATIONS Given the high value of our samples, we seek vendors that have been endorsed by Illumina and have demonstrable success with the platform. 3. SPECIFIC TASKS/REQUIREMENTS a. DNA extraction (buffycoat) b. Custom Nextera library generation c. Illumina HiSeq2500 Rapid runsequencing (150 cycle paired-end), per lane d. Bioinformatics Fastq generation and QC Line Item Estimated Quantity Unit Unit Cost Total DNA extraction (buffycoat) 200 EA Custom library generation 500 EA Illumina HiSeq2500 Rapid runsequencing (150 cycle paired-end), per lane 6 EA Bioinformatics Fastq generation and QC 6 EA This notice of intent is not a request for competitive proposals; however, any responsible source who believes they are capable of meeting the requirement may submit a capability statement to the contracting office no later than Monday, August 8, 2016 at 1:00 PM EST. Interest/capability statements may be sent to Linda Buccigrossi at [email protected]. No telephone responses will be accepted. A determination not to compete the proposed requirement based upon the responses to this notice is solely within the discretion of the Government. When submitting capability statement please identify in the email subject line the following: AAA Validation of Genome Sequencing - Palo Alto Response to Sources Sought 4. FACILITY: VA Palo Alto Health Care System (VAPAHCS)