Bio-Rad Quest 10 Plus Chromatography and BioFrac Fractionation Systems

The vendor shall provide an equipment of brand name or equal specification to the Bio-Rad Quest 10 Plus Chromatography System and a BioFrac Fractionation System. Equipment installation, software and 12 months warranty that includes parts and labor must be provided. NIAMS is seeking to acquire a fast protein liquid chromatography instrument of brand name or equal specification to the NIAMS Bio-Rad Quest 10 Plus Chromatography and BioFrac Fractionation Systems. In order to support existing research projects, the laboratory requires a Fast Protein Liquid Chromatography instrument (FPLC) with the following characteristics: Standalone with small footprint protein purification apparatus Allow for the purification of proteins, RNPs, and RNAs Be capable of handling multiple protein purification strategies, including gel filtration, IMAC, or ion-exchange chromatography Be programmable, automated operation Supports automated fractionation collection Be capable of conductive measurements Be modular for future expansions/increased capability Includes a high-resolution touchscreen to visualize results Create an accurate blended buffer gradient Allow flexible placement of valves and detectors to minimize flow path Allow user friendly setup of connections, e.g. by LED-guided plumbing Allow user-friendly extension and addition of modules, ideally through automated module discovery and easy application-based system setup Include a multiwavelength detection module for simultaneous four-wavelength detection at 190–800 nm (UV/VIS) Automated 10 ml/min pumps Supports flow rates of 0.001–10 ml/min at 3,650 psi Injection valve must multiple volume injections (263 to 750 microliters) Include a pre-installed machine control and analysis software Software must support instrument control, data collection and analysis OTHER REQUIREMENTS: Twelve months warranty PC Computer with 22-inch monitor Software License Instrument and Proteomics Training Microplate Rack with ice bath that holds 120 tubes, 12--13 mm in diameter; 4 SBS format microplates (96-, 48-, 24-, or 12-wells); Titertube and microtubes (8 x 12, 96-tubes)