Next-Gen Tabletop Sequencer

The United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Veterinary Services (VS), National Veterinary Science Lab (NVSL), Ames, IA, intends to issue a sole source purchase order with Illumina, Inc. for a Next-Gen Tabletop Sequencer, the MiSeq system. Specifications: 90% of bases with Q scores greater than 30 (1 × 35 bp)  >85% of bases with Q scores greater than 30 (2 x 100 bp )  >75% of bases with Q scores greater than 30 (2 x 150 bp )  >75% of bases with Q scores greater than 30 (2 x 250 bp) Light-emitting diode at 530 nm, 660 nm; Single flow cell system; Ability to stream data to BaseSpace, a cloud computing environment, eliminating the need for onsite data storage and dedicated analysis computers, providing instant, web-based data management, archival, analysis, and sharing capabilities; Low sample input of 1 ng for many genomic DNA samples. No need for emulsion PCR or additional pieces of equipment, amplification is performed directly on the instrument; A single operator can amplify up to 96 samples or more on a single channel flow cell in one hour using a single instrument; Automated data analysis using BWA/GATK for alignment and variant calling; Outputs standard file formats that are compatible with most third party software: fastq, bam, vcf and text (csv, tsv). The proposed contract action is for supplies for which the Government intends to solicit and negotiate with only one source under the authority of FAR 6.302-1, Only One Responsible Source and No Other supplies or Services will Satisfy Agency Requirements. This notice of intent is not a request for competitive proposals, nor is a solicitation available. A determination by the Government not to compete this proposed contract based on responses to this notice is solely at the discretion of the Government. Information received will be considered solely for information purpose and telephone requests will not be honored.