Barocycler NEP2320-45K

The United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Beltsville, MD, intends to issue a contract with one source, with that source being Pressure BioSciences, Inc., for a Barocycler NEP2320-45K, PBI Compressor, High Temperature Kit-90C, Shredder SG3 Kit and MicroTube TackleBox for NEP2320, for a total dollar amount of $36,110.00. The proposed contract action is for supplies for which the Government intends to solicit and negotiate with only one source under the authority of FAR 6.302. This equipment is for sample preparation in genomics, proteomics, transcriptomics and metabolomics and allows for the safe, rapid and reproducible extraction of DNA, RNA, proteins and small molecules from a wide variety of cells and tissues, particularly those considered hard to lyse. This is a programmable controlled pressure delivery system to sample chamber up to 45,000 psi, temperature control ranging from 4 to 50 degrees C; safety engineered sample containers for 10-1500uL liquid capacity including adaptor for solid sample; pressure delivery for 99 second hold or 99 consecutive cycles as quick as 20 seconds at elevated pressure and 5 seconds at ambient. The requirement is for sample preparation beyond homogenization - enhanced enzymology, organelle isolation/differential lysis. Specifications include a maximum pressure of 45,000 psi, pressure cycling ability of up to 99 cycles, pressure control of 5,000-35,000 psi in 1000 psi increments and temperature control range of 4C-50C. This notice of intent is not a request for competitive proposals. A determination by the Government not to compete with this proposed contract based upon responses to this notice is solely within the discretion of the Government.