Supplies for the Illumina NextSeq sequencer

The United States Department of Agriculture (USDA)/Animal Plant Health Inspection Service (APHIS)/ Plant Germplasm Quarantine Program (PGQP)/ in Beltsville, Maryland intends to award a sole source, Firm Fixed Price Purchase Order for Supplies for the Illumina NextSeq sequencer to Illumina, Inc. DUNS: 033305264 Cage 1WCS2 Supplies for the Illumina NextSeq sequencer Catalog number Description & Quantity 001 20020495 1(ea) AmpliSeq™ Custom DNA Panel for Illumina® Targeted custom with less than 4,999 amplicons panel optimized for specific targets or genomic content of interest. Library PLUS and index adapters sold separately. Project ID: 190847 Customer Product Name: iWG_PROJ_0002177_A-1 Species Name: PLANT_PATHOGEN Number of Amplicons: 3062 002 20019102 2(ea) AmpliSeq™ Library PLUS (96 Reactions) for Illumina® Reagents for fast amplicon library preparation with AmpliSeq™ panels, optimized for Illumi a sequencing systems. 003 20019105 1(ea) AmpliSeq™ CD Indexes Set A for Illumina® AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples) 004 20024906 5(ea) NextSeq 500/550 High Output Kit v2.5 (75 Cycles) Provides kitted reagents for 75 cycles of sequencing, plus dual-indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (75 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge. 005 20024905 1(ea) NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Mid Output Flow Cell Cartridge, Cartridge. 006 20020611 2(ea) TruSeq® Stranded Total RNA Library Prep Plant (96 Samples) This component contains the RiboZero beads to deplete cytoplasmic, mitochondrial, and chloroplast ribosomal RNA from plant samples as well as the PCR master mix to transcript RNA into cDNA for 96 samples. 007 20020610 (1ea) TruSeq® Stranded Total RNA Library Prep Plant (48 Samples) This component contains the RiboZero beads to deplete cytoplasmic, mitochondrial, and chloroplast ribosomal RNA from plant samples as well as the PCR master mix to transcript RNA into cDNA for 48 samples. 008 20022654 1(ea) Ampliseq™ cDNA Synthesis for Illumina® AmpliSeq™ cDNA Synthesis for Illumina® is required to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels. Examples of these panels include AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus and Comprehensive Panel v3 as well as Custom or Community RNA Panels. The product is supplied in a 2- tube format with the reaction mix and enzyme blend in separate tubes. Number of reactions varies by panel. For AmpliSeq for Illumina Immune Response, Focus and Comprehensive Panel v3 and Custom panels, 100 reactions per kit, and for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid and Immune Repertoire Plus, TCR beta Panels, 200 reactions per kit. 009 Plus Shipping & Insurance / Handling Fee This procurement is being conducted in accordance with Federal Acquisition Regulation (FAR) 6.302-1 (a)(See statutory authority 41 U.S.C. 253(c)(1). The property or services needed by the executive agency are available from only one responsible source and no other type of property or services will satisfy the needs of the executive agency. And 6.302-1(ii) (See statutory authority 41 U.S.C. 253 (d)(1)(B).) Supplies may be deemed to be available only from the original source in the case of a follow-on contract for the continued development or production of a major system or highly specialized equipment, including major components thereof, when it is likely that award to any other source would result in— (A) Substantial duplication of cost to the Government that is not expected to be recovered through competition; or (B) Unacceptable delays in fulfilling the agency’s requirements. Rationale or circumstances for limiting competition: The Illumina NextSeq 500 platform was originally purchased by the Plant Germplasm Quarantine Program in 2017. Illumina Inc is the only company that provides service for this NextSeq platform. The service covers remote technical support, next business day onsite response, on-site applications support, discounts on advanced training and remote monitoring. This Notice of Intent is not a request for competitive proposals. No solicitation documents are available and telephone requests will not be honored. However, if a vendor believes it can meet the requirements please contact the Contracting Officer by on or before the closing date of this notice. Supporting evidence must be furnished in sufficient detail to demonstrate the ability to comply with the above requirement. The North American Industry Classification System (NAICS) code for this acquisition is 334516 Analytical Laboratory Instrument Manufacturing , and the Small business size standard is 1,000 Employees. Responses received after the response date or without the required information will be deemed nonresponsive to the synopsis and will not be considered. Capability statements may be sent (via email only) to: Carol Dingess, Contracting Officer /[email protected].